Green Synthesis of Magnetic Nanoparticles from Oldenlandia diffusa: Characterization and Therapeutic Activities

This study investigated green synthesis of magnetic nanoparticles (MNP) using Oldenlandia diffusa , its cauterization, and therapeutic propertieson liver cancer cells via an in-vitro approach. Anti-cancer, anti-oxidant, and anti-diabetic properties of MNP were evaluated. Synthesis of MNP was confirmed via UV-visible spectroscopy. Oldenlandia diffusa MNP (20–40 nm) showed absorption peak at 327 nm in UV-visible, XRD, EDX and FTIR spectral analysis. MNP were monoclinic, and rhombohedral with two stretching peaks at 646 cm −1 , and 1098 cm −1 confirming Fe–O–Fe mode and Fe = O due to the iron element. IC 50 values for MNP (IC 50 74 μ g/ml & 58 μ g/ml) using DPPH and ABTS methods were high as compared to aqueous leaf extract of Oldenlandia diffusa confirming anti-oxidant properties. MNP exhibited α -amylase inhibitory activity with maximum inhibition of 82.17±0.82 μ g/ml for α -glucosidase inhibitory action of 84.82±0.89 μ g/ml. MNP showed dose-dependant in-vitro tumor suppression activity against HepG2 cells (16.08% at 150 μ g/ml and 28.31% at 150 μ g/ml). MNP also demonstrated anti-leishmanial activity at 400 g/ml (74.26±0.82 g/ml for promastigotes and 69.40±0.69 μ g/ml for amastigotes). Oldenlandia diffusa actively bonded with magnetic Fe-nanoparticles and suppressed the growth of HepG2 cells. It demonstrated anti-cancer, antioxidant, anti-leishmanaland anti-diabetic activities. Thus, Oldenlandia diffusa is a potential anti-cancer therapeutic drug for liver cancer.