Exploring bacterial extracellular vesicles: Focus on WHO critical priority pathogens

Bacterial extracellular vesicles (EVs) are cell-derived particles with a phospholipidic bilayer structure and diameter ranging from 20 to 250 nm, comprising a varied of components, including bioactive proteins, lipids, DNA, RNA, and other metabolites. These EVs play an essential role in bacterial and host function and are recognized as essential keys in cell-to-cell communication and pathogenesis. Due to these characteristics and functions, EVs exhibit great potential for biomedical applications and are promising tools for the development of drug delivery systems and vaccines, as well as for use in disease diagnostics. An interesting focus of this review is on the clinical relevance of EVs, with a particular emphasis on two critical pathogens, Acinetobacter baumannii and Klebsiella pneumoniae. Insights into the outer membrane vesicles (OMVs) derived from these bacteria underscore their roles in antimicrobial resistance and pathogenicity. Additionally, the review explores OMV-based vaccine strategies as a promising means to mitigating these pathogens.