Probing Twist‐Induced Endocytotic Membrane Fission using Anisotropic Gold Homodimers

Abstract Membrane fission involves a crucial step of lipid remodeling, in which the dynamin collar constricts and severs the tubulated lipid membrane at the neck of budding vesicles. Nevertheless, the difficulty in accurately determining the rotational dynamics of live endocytotic vesicles poses a limit on the elucidation of dynamin‐induced membrane remodeling for endocytotic vesicle scission. Herein, we designed a DNA‐modified gold homodimer (AuHD)‐based anisotropic plasmonic probe with uniform surface chemistry, minimizing orientational fluctuation within vesicle encapsulation. Using AuHDs as cargos to image the dynamics of cargo‐containing vesicles during endocytosis, we showed that, prior to detachment from plasma membrane, the cargo‐containing vesicles underwent multiple intermittent twists of ~4° angular orientation relative to plasma membrane with a ~0.2 s dwell time. These findings suggest that the membrane torques resulting from dynamin actions in vivo constitute the pathway to membrane fission, potentially shedding light on how dynamin‐mediated lipid remodeling orchestrates membrane fission.