生物
Wnt信号通路
转染
过氧化物酶体增殖物激活受体
细胞生长
核受体
信号转导
分子生物学
癌症研究
癌变
受体
细胞培养
细胞生物学
转录因子
基因
生物化学
遗传学
作者
Fang Guo,Xiyun Ren,Yingzi Dong,Xiaomeng Hu,Dan Xu,Haibo Zhou,Fengyan Meng,Weijun Tian,Yong Zhao
出处
期刊:Gene
[Elsevier]
日期:2016-06-01
卷期号:584 (1): 31-37
被引量:29
标识
DOI:10.1016/j.gene.2016.03.003
摘要
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the PPAR nuclear hormone receptor superfamily, which plays a crucial role in carcinogenesis. Wnt/β-Catenin signaling pathway has been well certified to contribute to the progression of gastric malignancies. β-Catenin mediates transcriptional regulation by forming a complex with LEF/TCF transcription factors, resulting in activation of downstream target genes such as TERT, ENAH. In this study, we aimed at detecting the effect of PPARγ on TERT, ENAH and explaining the further mechanisms of PPARγ on tumor suppression. The pEGFP-N1-PPARγ recombinant plasmid has already been constructed by researchers in our laboratory. We stably transfected it into three gastric cancer (GC) cell lines (MKN-28, SGC-7901 and BGC-823). CCK-8 and transwell assay were employed to analyze the capability of cell proliferation and metastasis. The mRNA and protein levels were evaluated by real-time PCR and western blot analysis. After transfected with PPARγ overexpression plasmid, the ability of cell proliferation and migration declined significantly (p < 0.05). The expression of PPARγ increased (p < 0.05) and β-Catenin was inhibited obviously (p < 0.05) in the group of pEGFP-N1-PPARγ plasmid transfection. Meanwhile, the mRNA or protein levels of TERT and ENAH were suppressed (p < 0.05) in pEGFP-N1-PPARγ plasmid transfection group compared with control groups. PPARγ might inhibit the proliferation and migration of GC cell lines through suppressing the expression of TERT and ENAH. PPARγ played an important role as a physiological regulator and might be a target for the treatment of GC.
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