化学
门控
孔蛋白
分析物
纳米孔
生物物理学
细胞外
色谱法
细菌外膜
生物化学
纳米技术
生物
基因
材料科学
大肠杆菌
作者
Monifa A. Fahie,Bib Yang,Martin Mullis,Matthew A. Holden,Min Chen
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2015-10-23
卷期号:87 (21): 11143-11149
被引量:59
标识
DOI:10.1021/acs.analchem.5b03350
摘要
Outer membrane protein G is a monomeric β-barrel porin that has seven flexible loops on its extracellular side. Conformational changes of these labile loops induce gating spikes in current recordings that we exploited as the prime sensing element for protein detection. The gating characteristics, open probability, frequency, and current decrease, provide rich information for analyte identification. Here, we show that two antibiotin antibodies each induced a distinct gating pattern, which allowed them to be readily detected and simultaneously discriminated by a single OmpG nanopore in the presence of fetal bovine serum. Our results demonstrate the feasibility of directly profiling proteins in real-world samples with minimal or no sample pretreatment.
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