The Optimization Study on Solid Culture Medium of North Cordyceps

Artificial cultivation of solid culture medium of North Cordyceps was optimized in this experiment. The results show that, the North Cordyceps can be cultivated in many types of solid culture medium, the growth rate and density of the hyphae was highest in a single solid culture medium with rice as the solid substrate, but adding 10%-15% wheat bran in rice solid culture medium, the growth rate and density of the hyphae is better than the single matrix of rice; the suitable nutrient solution was determined by contrast test and L9 (34) orthogonal test, the composition was sucrose 2%, yeast extract 1.5%, MgSO 4 0.1%, KH 2 PO4 0.1%; the suitable solid culture medium of the North Cordyceps sinensis was determined by the L9 (33) orthogonal test, the composition was rice 85%, wheat bran 15%, pH 5.5-6.5, the ratio of solid and water ( solid matrix / nutrient solution )was 1:1.5.