生物
抗体
噬菌体展示
抗体库
丝状噬菌体
抗原
噬菌体
噬菌体
分子生物学
免疫球蛋白轻链
半抗原
基因
病毒学
大肠杆菌
生物化学
遗传学
作者
Greg Winter,Andrew D. Griffiths,Robert E. Hawkins,Hennie R. Hoogenboom
出处
期刊:Annual Review of Immunology
[Annual Reviews]
日期:1994-04-01
卷期号:12 (1): 433-455
被引量:1478
标识
DOI:10.1146/annurev.iy.12.040194.002245
摘要
Antibody fragments of predetermined binding specificity have recently been constructed from repertoires of antibody V genes, bypassing hybridoma technology and even immunization. The V gene repertoires are harvested from populations of lymphocytes, or assembled in vitro, and cloned for display of associated heavy and light chain variable domains on the surface of filamentous bacteriophage. Rare phage are selected from the repertoire by binding to antigen; soluble antibody fragments are expressed from infected bacteria; and the affinity of binding of selected antibodies is improved by mutation. The process mimics immune selection, and antibodies with many different binding specificities have been isolated from the same phage repertoire. Thus human antibody fragments have been isolated with specificities against both foreign and self antigens, including haptens, carbohydrates, secreted and cell surface proteins, viral coat proteins, and intracellular antigens from the lumen of the endoplasmic reticulum and the nucleus. Such antibodies have potential as reagents for research and in therapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI