生物
胚胎干细胞
干细胞
多效蛋白
期限(时间)
细胞生物学
遗传学
生长因子
基因
受体
物理
量子力学
作者
Boon-Seng Soh,Chun Meng Song,Ludovic Vallier,Pin Li,Cleo Choong,Boon Huat Yeo,Elaine Hsuen Lim,Roger A. Pedersen,Henry Yang,Mahendra S. Rao,Bing Lim
出处
期刊:Stem Cells
[Oxford University Press]
日期:2007-09-06
卷期号:25 (12): 3029-3037
被引量:57
标识
DOI:10.1634/stemcells.2007-0372
摘要
Abstract To identify additional growth factors for optimizing propagation of human embryonic stem cells (hESCs), we mined publicly available data sets for the transcriptomes of murine and human ESCs and feeder cells, thereby generating a list of growth factors and complementary receptors. We identified the major pathways previously reported to be important, as well as several new ones. One pathway is the Pleiotrophin (PTN)-Pleiotrophin receptor (PTPRZ1) axis. Murine fibroblasts secrete Ptn, whereas hESCs expressed PTPRZ1, which is downregulated upon differentiation. Depletion of PTPRZ1 resulted in decreased colony formation and lower recovery of hESCs. Supplementation of chemically defined medium for feeder-free propagation of hESCs with PTN allowed higher recovery of hESCs without loss of pluripotency. PTN-PTPRZ1 functions here predominantly via an antiapoptotic effect mediated in part by the activation of Akt. These findings reveal the underlying importance of PTN in hESC survival and its usefulness in the clonal manipulation and large-scale propagation of hESCs. Disclosure of potential conflicts of interest is found at the end of this article.
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