脂质体
蓖麻毒素
细胞毒性
莫能星
叶酸受体
化学
生物化学
体外
毒素
生物
癌细胞
癌症
遗传学
作者
Nidhi Tyagi,Prahlad C. Ghosh
标识
DOI:10.1016/j.ejps.2011.05.010
摘要
Ricin was encapsulated into various sterically stabilized liposomes having different density of folate on the surface and the cytotoxicity of ricin in these liposomes was examined in KB cells. The effect of monensin in free and various sterically stabilized liposomal forms having different density of folate on the surface on the enhancement of cytotoxicity of ricin entrapped in these liposomes was also examined. It was observed that liposomal ricin having 0.5 mol% folate–PEG on the surface exhibits maximum cytotoxicity (IC50 = 1274 ng/ml) in KB cells as compared to non-targeted liposomes (IC50 = 3274 ng/ml). Monensin either in free form (266.2-fold) or liposomal form (291.5-fold) enhances the cytotoxicity of this targeted liposomal ricin significantly. This enhancement of the cytotoxicity of ricin entrapped in folate-targeted liposomes is further enhanced to 557.7-fold by monensin when it was delivered through folate-targeted (0.5 mol% folate–PEG) liposomes. The present study has clearly demonstrated that ricin entrapped in folate-tagged-sterically stabilized liposomes in combination with monensin intercalated in folate-tagged-sterically stabilized liposomes may have potential application for the treatment of cancer cells over-expressing folate receptors on the cell surface.
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