光动力疗法
光毒性
介孔二氧化硅
纳米载体
材料科学
乙二醇
聚乙烯亚胺
生物相容性
光敏剂
生物物理学
纳米颗粒
PEG比率
介孔材料
纳米技术
化学
光化学
体外
有机化学
转染
生物化学
基因
催化作用
经济
冶金
生物
财务
作者
Jing Tu,Tianxiao Wang,Wei Shi,Guisen Wu,Xin Tian,Yuhua Wang,Dongtao Ge,Lei Ren
出处
期刊:Biomaterials
[Elsevier]
日期:2012-11-01
卷期号:33 (31): 7903-7914
被引量:115
标识
DOI:10.1016/j.biomaterials.2012.07.025
摘要
The cellular uptake and localization of photosensitizer-loaded nanoparticles have significant impact on photodynamic therapy (PDT) efficacy due to short lifetime and limited action radius of singlet oxygen. Herein, we develop poly(ethylene glycol) (PEG)- and polyethylenimine (PEI)-functionalized zinc(II) phthalocyanine (ZnPc)-loaded mesoporous silica nanoparticles (MSNs), which are able to distribute in the cytosol by endolysosomal escape. In this photosensitizer-carrier system (PEG-PEI-MSNs/ZnPc), ZnPc is a PDT agent; MSNs are the nanocarrier for encapsulating ZnPc; PEI facilitates endosomal escape; and PEG enhances biocompatibility. The as-synthesized PEG-PEI-MSNs/ZnPc have a high escape efficiency from the lysosome to the cytosol due to the "proton sponge" effect of PEI. Compared with the ZnPc-loaded MSNs, the phototoxicity of the PEG-PEI-MSNs/ZnPc is greatly enhanced in vitro. By measuring the mitochondrial membrane potential, a significant loss of >80% Δψm after treatment with PEG-PEI-MSNs/ZnPc-PDT is observed. It is further demonstrated that the ultra-efficient passive tumor targeting and excellent PDT efficacy are achieved in tumor-bearing mice upon intravenous injection of PEG-PEI-MSNs/ZnPc and the followed light exposure. We present here a strategy for enhancement of PDT efficacy by endolysosomal escape and highlight the promise of using multifunctional MSNs for cancer therapy.
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