促炎细胞因子
埃利斯波特
子痫前期
免疫学
医学
炎症
生物
怀孕
T细胞
免疫系统
遗传学
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 101-108
标识
DOI:10.1007/978-1-0716-2903-1_8
摘要
The main objective of this chapter is to determine inflammatory and anti-inflammatory cytokines in patients with or without preeclampsia by means of the enzyme-linked immunosorbent assay (ELISA) technique. In this chapter, 16 cell cultures were obtained from different patients admitted to the hospital with term vaginal delivery or cesarean section. Here, we describe the ability to quantify the presence of cytokines in cell culture supernatants. The supernatants of the cell cultures were collected and concentrated. The concentration of IL-6 and VEGF-R1 was measured by ELISA to determine the prevalence of alterations in the samples studied. We observed that the sensitivity of the kit allowed the detection of several cytokines in a range between 2 and 200 pg/mL. The test was performed using the ELISpot method (5×), which allowed a higher level of precision.
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