纳米器件
小RNA
DNA
上皮-间质转换
癌细胞
纳米技术
癌症
细胞生物学
计算生物学
化学
材料科学
生物
过渡(遗传学)
基因
生物化学
遗传学
作者
Youming Feng,Juanjuan Liu,Manli Tan,Lele Li,Ming Gao,Jian Zhao,Li Zheng
出处
期刊:Nano Today
[Elsevier]
日期:2023-06-01
卷期号:50: 101881-101881
标识
DOI:10.1016/j.nantod.2023.101881
摘要
Despite microRNAs (miRNAs) are key regulators of epithelial-mesenchymal transition (EMT), approaches allowing for real-time monitoring of EMT process of tumor cells via miRNA imaging have yet to be achieved. Herein we present the design of an enzyme-controlled DNA nanodevice for activatable parallel imaging of two independent target miRNAs, thus achieving cancer cell-specific monitoring of EMT process. Toehold-mediated strand displacement reactions and enzyme activatable modules are integrated in the DNA nanodevice design to achieve generation of multiple signal outputs in response to different miRNA inputs. The system allows for enzymatically activatable two-input and three-output imaging patterns in cancer cells, while possesses no miRNA sensing functions in normal cells, resulting in an enhanced sensitivity and cell specificity for the cancer-selective monitoring of EMT dynamics. Furthermore, it is demonstrated that the DNA nanodevice can rapidly discriminate EMT state of chemoresistant carcinoma cells by duplexed miRNA imaging in an enzyme-activated manner. We envision the application of this multiplexed imaging technology for decoding the roles of EMT in diverse pathological processes.
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