基质(水族馆)
生物复合材料
辣根过氧化物酶
酶
材料科学
金属有机骨架
多孔性
连接器
化学工程
结晶
催化作用
固定化酶
化学
组合化学
复合材料
有机化学
吸附
复合数
海洋学
计算机科学
工程类
地质学
操作系统
作者
Emily Massahud,Heba Ahmed,Lizebona August Ambattu,Amgad R. Rezk,Leslie Yeo
标识
DOI:10.1016/j.mtchem.2023.101694
摘要
Encapsulating enzymes within metal–organic frameworks (MOFs) constitute a promising strategy to circumvent their fragile nature in harsh environments, although deleterious molecular conformational changes and decreased substrate accessibility within the pores of the MOF during enzyme loading can often result in decreased catalytic activity. Herein, we report a rapid (within seconds) and facile one-pot aerosol-based method for simultaneous MOF crystallisation and enzyme encapsulation for the synthesis of ZIF-8/horseradish peroxidase (HRP) biocomposites using a high frequency acoustomicrofluidic nebulisation platform. As a consequence of the enhanced mixing and fast crystallisation afforded by the acoustic coupling into the precursors, the resultant combination of enhanced porosity, missing-linker/metal-node defect creation, pore activation and favourable conformational changes to the enzyme secondary structure allows for more efficient enzyme loading and greater enzyme–substrate interactions, and hence stronger enzyme binding within the MOF structure. Together, these culminate in a biocomposite that possesses four times greater enzymatic activity compared to that for the same material synthesized using conventional bulk-solution techniques, and endows the enzyme with increased protective effect (by approximately three to seven times) against high temperature and organic solvents, even after several exposure cycles. Such a possibility facilitates better potential for the enzyme to be recycled—an important factor given their typically high costs and the difficulty in recovering free enzymes in solution.
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