Iron addition promotes mercury removal from soil by Robinia pseudoacacia–rhizobia symbiosis

Abstract Iron plaques on the root surface can promote or inhibit the absorption and accumulation of heavy metals by plants. However, the mechanism by which iron regulates the response of Robinia pseudoacacia to mercury (Hg) has not been elucidated, which hinders its application in divalent Hg (Hg2+) removal from Hg-contaminated soil. In this study, association analyses between transcriptome and metabolome were used to investigate effects of iron on the rhizosphere microenvironment and performance of R. pseudoacacia to assess its potential for Hg2+ removal. The results showed that the addition of 10 mg kg−1 iron significantly increased the development of iron plaques on the root surface and reduced the secretion of low-molecular-weight organic acids by roots, thereby changing rhizosphere soil characteristics and decreasing total Hg in roots. In addition, the secretion of choline supported signal transduction and enhanced the interaction between R. pseudoacacia and rhizobia, thereby inducing resistance to Hg2+. Anti-oxidative enzyme activities were increased and Hg2+ exposure of plants was reduced. Enhanced Hg2+ resistance was indicated by improved photosynthesis and growth, despite promoted xylem loading and transport of Hg2+, resulting in its accumulation in aboveground tissues, which is essential for Hg2+ removal. These results indicate that iron addition has a great potential to improve the growth of R. pseudoacacia in Hg-contaminated soil and promote the accumulation of Hg2+ in aboveground tissues for phytoremediation approaches.