Elevated EBF2 in mouse but not pig drives the progressive brown fat lineage specification via chromatin activation

Brown adipose tissue (BAT) is responsible for non-shivering thermogenesis, but it is absent in some mammals, including pigs. During development, BAT progenitors are derived from paired box 7 (Pax7)-expressing somitic mesodermal stem cells, which also give rise to skeletal muscle. However, the intrinsic mechanisms underlying the fate decisions between brown fat and muscle progenitors remain elusive. In this study, we analyzed the dynamics of chromatin landscape during the segregation of specification of brown fat and muscle lineages from Pax7