三七
生物
茉莉酸
WRKY蛋白质结构域
索拉尼镰刀菌
转录因子
植物抗病性
水杨酸
基因
基因表达
转录组
细胞生物学
遗传学
植物
病理
替代医学
医学
作者
Linlin Su,Lilei Zheng,Hanlin Wang,Yuan Qu,Feng Ge,Diqiu Liu
标识
DOI:10.1186/s12870-023-04373-x
摘要
Abstract Background Panax notoginseng (Burk) F. H. Chen is a valuable traditional Chinese medicinal plant, but its commercial production is seriously affected by root rot caused by some pathogenic fungi, including Fusarium solani . Nevertheless, the genetic breeding for disease resistance of P. notoginseng remains limited. The WRKY transcription factors have been revealed to play important roles in plant defense responses, which might provide an inspiration for resistance improvement in P. notoginseng . Results In this study, the regulatory mechanism of transcription factor PnWRKY15 on P. notoginseng resistance to F. solani infection was revealed. The suppressed expression of PnWRKY15 via RNA interference increased the sensitivity of P. notoginseng to F. solani and decreased the expression levels of some defense-related genes, including PnOLP1 , which encodes an osmotin-like protein that confers resistance to F. solani . Ectopic expression of PnWRKY15 in the model plant tobacco significantly enhanced the resistance to F. solani . Moreover, the transcriptome sequencing analysis discovered that some pathogenesis-related genes were expressed at higher levels in the PnWRKY15 -overexpressing tobacco than that in the wild-type tobacco. In addition, the jasmonic acid (JA) and salicylic acid (SA) signaling pathways were evidently induced by PnWRKY15 -overexpression, that was evidenced by that the JA and SA contents were significantly higher in the PnWRKY15 -overexpressing tobacco than that in the wild-type. Furthermore, PnWRKY15, which was localized in the nucleus, can trans-activate and up-regulate PnOLP1 expression according to the EMSA, yeast one-hybrid and co-expression assays. Conclusions PnWRKY15 contributes to P. notoginseng resistance to F. solani by up-regulating the expression of resistance-related gene PnOLP1 and activating JA/SA signaling pathways. These findings will help to further elucidate the transcriptional regulatory mechanism associated with the P. notoginseng defense response to F. solani .
科研通智能强力驱动
Strongly Powered by AbleSci AI