生物传感器
检出限
化学
聚合酶链反应
组合化学
生物标志物
计算生物学
纳米技术
分子生物学
生物
材料科学
生物化学
色谱法
基因
作者
Moli Zhang,Yang Yang,Lingyi Xin,Hua Zhang,Lun Wu,Jun Zhu,Jing Zhu,Shiyun Liu,Zhaohui Wang,Qinhua Chen,Guangyi Yang
出处
期刊:Molecules
[Multidisciplinary Digital Publishing Institute]
日期:2023-02-27
卷期号:28 (5): 2208-2208
被引量:5
标识
DOI:10.3390/molecules28052208
摘要
Recently, miRNAs have become a promising biomarker for disease diagnostics. miRNA-145 is closely related to strokes. The accuracy determination of miRNA-145 (miR-145) in stroke patients still remains challenging due to its heterogeneity and low abundance, as well as the complexity of the blood matrix. In this work, we developed a novel electrochemical miRNA-145 biosensor via subtly coupling the cascade strand displacement reaction (CSDR), exonuclease III (Exo III), and magnetic nanoparticles (MNPs). The developed electrochemical biosensor can quantitatively detect miRNA-145 ranging from 1 × 102 to 1 × 106 aM with a detection limit as low down as 100 aM. This biosensor also exhibits excellent specificity to distinguish similar miRNA sequences even with single-base differences. It has been successfully applied to distinguish healthy people from stroke patients. The results of this biosensor are consistent with the results of the reverse transcription quantitative polymerase chain reaction (RT-qPCR). The proposed electrochemical biosensor has great potential applications for biomedical research on and clinical diagnosis of strokes.
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