Cross-Reactive Carbohydrate Determinants (CCDs) Causing Interference in Allergen-Specific IgE Antibody Analysis

BACKGROUND: The use of CCD antibody adsorbents specifically adsorb CCDs, reducing non-specific IgE interference in chemiluminescence assays, thereby making the detection results of true allergen-specific IgE more accurate. METHODS: Chemiluminescence assays were used to quantitatively detect allergens (total IgE and specific IgE) in patient serum, with heterophile antibody removers to exclude the interference of heterophile antibodies, and anti-CCD IgE adsorbents to exclude the interference of CCDs. RESULTS: The detection results after using allergen heterophile antibody removers showed no difference from the original results, while the specific IgE returned to normal after using CCD-specific IgE adsorbents, with no significant change in total IgE. CONCLUSIONS: When multiple allergen sIgE are positive simultaneously, or when the allergen detection results do not match the patient's clinical symptoms, history of allergen exposure, or skin test results, it is necessary to perform anti-CCD IgE adsorption tests to improve the accuracy of allergen diagnosis.