This paper presents a pilot study that uses a markerless motion capture system to measure active shoulder joint range of motion in a clinic room. The measurements from this automated method are compared with manual measurements done by a clinician using a goniometer. All the parameters from both methods are positively correlated. The root-mean-square differences from the comparisons are in the range of 7.21° – 15.87° depending on the type of angles. With a careful protocol design and execution, the markerless motion capture system can potentially be used as an objective shoulder range of motion assessment tool in clinical practice.