远臂足
短柄草属
氮气
铵
植物
营养物
化学
生物量(生态学)
氮缺乏
生物
生物化学
农学
有机化学
基因组
基因
作者
Vlastimil Novák,Benjamin P. Bowen,Benjamin P. Bowen,Yezhang Ding,Kateryna Zhalnina,Kirsten Hofmockel,Connor Tomaka,Thomas V. Harwood,Michelle C. M. van Winden,Amber Golini,Suzanne M. Kosina,Trent Northen
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2024-01-05
卷期号:10 (1)
标识
DOI:10.1126/sciadv.adg7888
摘要
Understanding plant-microbe interactions requires examination of root exudation under nutrient stress using standardized and reproducible experimental systems. We grew Brachypodium distachyon hydroponically in fabricated ecosystem devices (EcoFAB 2.0) under three inorganic nitrogen forms (nitrate, ammonium, and ammonium nitrate), followed by nitrogen starvation. Analyses of exudates with liquid chromatography–tandem mass spectrometry, biomass, medium pH, and nitrogen uptake showed EcoFAB 2.0’s low intratreatment data variability. Furthermore, the three inorganic nitrogen forms caused differential exudation, generalized by abundant amino acids–peptides and alkaloids. Comparatively, nitrogen deficiency decreased nitrogen-containing compounds but increased shikimates-phenylpropanoids. Subsequent bioassays with two shikimates-phenylpropanoids (shikimic and p -coumaric acids) on soil bacteria or Brachypodium seedlings revealed their distinct capacity to regulate both bacterial and plant growth. Our results suggest that (i) Brachypodium alters exudation in response to nitrogen status, which can affect rhizobacterial growth, and (ii) EcoFAB 2.0 is a valuable standardized plant research tool.
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