噬菌体
纳米技术
大肠杆菌
微生物学
微型多孔材料
细菌
多重耐药
材料科学
化学
生物
抗药性
遗传学
生物化学
基因
复合材料
作者
Lei Tian,Leon He,Kyle Jackson,Ahmed Saif,Shadman Khan,Zeqi Wan,Tohid F. Didar,Zeinab Hosseinidoust
标识
DOI:10.1038/s41467-022-34803-7
摘要
Abstract Nanofilamentous bacteriophages (bacterial viruses) are biofunctional, self-propagating, and monodisperse natural building blocks for virus-built materials. Minifying phage-built materials to microscale offers the promise of expanding the range function for these biomaterials to sprays and colloidal bioassays/biosensors. Here, we crosslink half a million self-organized phages as the sole structural component to construct each soft microgel. Through an in-house developed, biologics-friendly, high-throughput template method, over 35,000 phage-built microgels are produced from every square centimetre of a peelable microporous film template, constituting a 13-billion phage community. The phage-exclusive microgels exhibit a self-organized, highly-aligned nanofibrous texture and tunable auto-fluorescence. Further preservation of antimicrobial activity was achieved by making hybrid protein-phage microgels. When loaded with potent virulent phages, these microgels effectively reduce heavy loads of multidrug-resistant Escherichia coli O157:H7 on food products, leading to up to 6 logs reduction in 9 hours and rendering food contaminant free.
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