褪黑素
内分泌学
内科学
卵巢
氧化应激
PI3K/AKT/mTOR通路
生物
排卵
毛囊
卵泡发生
卵泡期
免疫印迹
激素
卵泡
男科
细胞生物学
信号转导
医学
胚胎发生
胚胎
基因
生物化学
作者
Yasemin Behram Kandemir,Çiğdem Aydın,Gökhan Görgişen
出处
期刊:Cellular and Molecular Biology
日期:2017-02-28
卷期号:63 (2): 100-100
被引量:34
标识
DOI:10.14715/cmb/2017.63.2.16
摘要
Mammalian Target of Rapamycin (mTOR) signaling pathway has important roles in the regulation of puberty onset, gonadotropin secretion, follicular development and ovulation. Melatonin (N-acetyl-5-methoxytryptamine) is a lipophilic hormone has multiple functions in regulating the fertility. Recent studies have shown that melatonin affected the number or maturation of follicles in the ovary. The aim of this study was to investigate the effects of melatonin on mTOR expression and quantity of follicle in rat ovary. In the present study, a total of 45 female rats were randomly divided into three groups. Group 1; Control (C), Group 2: Vehicle (V) and Group 3; Melatonin (M). Melatonin was administered intraperitoneally at a dose of 50 mg/kg/day for 30 days in Melatonin group. The effects of Melatonin on the expression of mTOR and downstream components were determined by Western Blot and Reverse Transcriptase PCR analysis. Upon Western Blot and RT-PCR evaluations, we detected higher expression and activation of mTOR, P70S6K, PKCalpha, PCNA and higher numbers of primordial follicles in melatonin group compared with V and C group. In addition to this results, melatonin decreased oxidative stress markers, such as MDA, on the contrary, levels of antioxidative markers, such as CAT and GPx, were increased by melatonin in rat ovary. This study indicated that melatonin may have a significant protective effect on primordial follicles and increase the expression of mTOR and downstream components in rat ovary. Melatonin treatment may have a beneficial effect on fertility.
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