To investigate the prevalence of Canine distemper virus(CDV) and Canine parvovirus(CPV) in Shanghai,samples were collected from 106 diseases dogs and 232 clinical healthy dogs and tested using Real-time fluorescent PCR.The positive rates of diseased dog samples were 50.0% and 72.6% for CDV and CPV,respectively.In clinical healthy samples,the CDV and CPV positive rates were 4.74% and 3.88%,respectively.These investigations illustrated the prevalence of CDV and CPV in dogs in Shanghai area.