Analysis of flavonoids in Flemingia philippinensis Merr. et Rolfe. by UPLC/Q-TOF-MS

AIM To analyze flavonoids in Flemingia philippinensis Merr.et Rolfe.,using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC/Q-TOF-MS).METHODS The chromatographic separation was performed on BEH C18 column with gradient elution of water(A)-acetonitrile(B).The mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive and negative ion modes.RESULTS Twenty flavonoids were identified based on their elemental compositions,MS/MS data with reference and published literatures within 10 min.CONCLUSION UPLC/Q-TOF-MS is a rapid and effective technology to obtain the structural information and the MS fragmentation regularity of flavonoids in Flemingia philippinensis Merr.et Rolfe.