Comparison of Three Methods for Evaluation on Cytotoxicity of Medical Products

Objective To compare three methods(MTT assay,living cells count and morphologic observation) for evaluation on cytotoxicity of medical products.Methods MTT assay,living cells count and morphologic observation were used to evaluate the cytotoxicity of nineteen pieces of medical products by cytotoxicity graduation and judgement of qualification,the results of these methods were analysed for degree of coincidence and comparison of advantages and disadvantages among these methods.Results The rate of coincidence of cytotoxicity graduation between MTT assay and living cells count was 73.7%(14/19) and that of qualification was 94.7%(18/19),the sample No.15 was tested four times with three methods,and the results show the difference between MTT assay and living cells-count were great,while the results of morphologic observation method were concordant with that of living cells count.Conclusion MTT assay is more suitable for evaluation of cytotoxicity of medical products for it is easy to operate and its errors are small,but living cells-count method and morphologic observation are needed to make a comprehensive judgement to unqualified medical products.