The temperature correlation of ultrasound attenuation coefficient in the organization of microwave hyperthermia is researched in order to achieve non-invasive organization temperature. Microwave heating experiments are carried out in vitro pig liver tissues, and ultrasonic echo signals are collected at different temperatures. The ultrasound attenuation coefficient in vitro liver are calculated at different temperatures, and temperature dependence of ultrasound attenuation coefficient are confirmed. The experiment results show that ultrasonic attenuation coefficient and temperature has a strong correlation (correlation coefficient is 0.5 or above), and there is a high temperature measurement accuracy (temperature error range is 0.2-4 ℃)based on the method of using the temperature dependence of ultrasonic attenuation coefficient to detect organization temperature in microwave hyperthermia.