Preparation and Regeneration of Protoplasts of Sporisorium reilianum f.

To establish protoplast mediated genetic transformation,preparation and regeneration of protoplasts from Sporisorium reilianum f.were studied.The results showed that the optional condition for the protoplast preparation was that,mating type strains SR1 were incubated in the mixed enzyme liquid consisting of 5 mg / mL lysing enzymes,50 mg / mL driselase,50 mg / mL snailase at 28 ℃ with shaking at 100 r / min for 10 min,and the yield of protoplast reached 1.35×108mL and the highest production rate of protoplast reached 99.36%.The protoplast was regenerated on the CM plates containing 1.0 mol / L D-sorbitol and the highest regeneration rate could reach 35.63%.