New Stability Indicating RP-UFLC Method for the Determination of Bumetanide – A Potent Diuretic

Bumetanide is a potent loop diuretic.A new ultrafast liquid chromatographic method has been developed for the assay and stability studies of Bumetanide in tablets and validated.Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector with C18 column (250 mm × 4.60 mm i.d.5µm particle size) was used with a mobile phase composition acetic acid: acetonitrile: water (0.1: 80: 20 v/v) and flow rate of 1.2 mL/min (UV detection at 220nm).Bumetanide has shown linearity 0.1-100 μg/mL with linear regression equation y = 70867x+3608 (r2 = 0.9996).The LOQ was found to be 0.0964 μg/mL and the LOD was found to be 0.03091 μg/mL.Bumetanide was subjected to forced degradation and the method was validated as per ICH guidelines.