间充质干细胞
细胞生物学
微泡
再生医学
细胞外小泡
间质细胞
胞外囊泡
干细胞
化学
生物
旁分泌信号
细胞疗法
骨髓
再生(生物学)
作者
Shalmali Pendse,Vaijayanti Kale,Anuradha Vaidya
出处
期刊:Current Stem Cell Research & Therapy
日期:2021-01-01
卷期号:16 (3): 243-261
被引量:2
标识
DOI:10.2174/1574888x15999200918110638
摘要
Mesenchymal stromal cells (MSCs) regulate other cell types through a strong paracrine component called the secretome, comprising of several bioactive entities. The composition of the MSCs' secretome is dependent upon the microenvironment in which they thrive, and hence, it could be altered by pre-conditioning the MSCs during in vitro culture. The primary aim of this review is to discuss various strategies that are being used for pre-conditioning of also known as of MSCs, in the context of improving their therapeutic potential. Several studies have underscored the importance of extracellular vesicles (EVs) derived from primed MSCs in improving their efficacy in the treatment of various diseases. We have previously shown that co-culturing hematopoietic stem cells (HSCs) with hypoxiaprimed MSCs improves their engraftment potential. Now the question we pose is would priming of MSCs with hypoxiafavorably alter theirsecretome and would this altered secretome work as effectively as the cell to cell contact did? Here we review the current strategies of using the secretome, specifically the EVs (microvesicles and exosomes), collected from the primed MSCs with the intention of expanding HSCs ex vivo. We speculate that an effective priming of MSCs in vitrocould modulate the molecular profile of their secretome, which could eventually be used as a cell-free biologic in clinical settings.
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