CO2 concentration affects in vitro pig embryo developmental capacity

Culture gas atmosphere is one of the most important factors affecting embryo development in vitro.The main objective of this study was to compare the effects of CO 2 concentration on the subsequent pre-implantation developmental capacity of pig embryos in vitro, including embryos obtained via parthenogenesis, in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI).Pig embryos were developed in four different CO 2 concentrations in air: 3%, 5%, 10%, or 15%.The cleavage rate of pig parthenogenetic, IVF, or ICSI embryos developed in CO 2 concentrations under 5% was the highest.There were no significant differences in the oocyte cleavage rate in ICSI embryos in CO 2 concentrations under 3% and 5% (p>0.05).However, as CO 2 levels increased (up to 15%) the blastocyst output on day 7, from parthenogenetic, IVF, and ICSI embryos, decreased to 0%.These findings demonstrate that CO 2 positively affects the developmental capacity of pig embryos.However, high or low CO 2 levels do not significantly improve the developmental capacity of pig embryos.The best results were obtained for all of the pig embryos at a 5% CO 2 concentration.