生物
染色质
拟南芥
细胞生物学
光周期性
转录因子
韧皮部
植物
基因
遗传学
突变体
作者
Tian Hao,Yuru Li,Ce Wang,Xingwen Xu,Yajie Zhang,Qudsia Zeb,Johan Zicola,Yong-Fu Fu,Franziska Turck,Legong Li,Zefu Lu,Liangyu Liu
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2021-01-04
卷期号:33 (3): 475-491
被引量:24
标识
DOI:10.1093/plcell/koaa043
摘要
Photoperiod plays a key role in controlling the phase transition from vegetative to reproductive growth in flowering plants. Leaves are the major organs perceiving day-length signals, but how specific leaf cell types respond to photoperiod remains unknown. We integrated photoperiod-responsive chromatin accessibility and transcriptome data in leaf epidermis and vascular companion cells of Arabidopsis thaliana by combining isolation of nuclei tagged in specific cell/tissue types with assay for transposase-accessible chromatin using sequencing and RNA-sequencing. Despite a large overlap, vasculature and epidermis cells responded differently. Long-day predominantly induced accessible chromatin regions (ACRs); in the vasculature, more ACRs were induced and these were located at more distal gene regions, compared with the epidermis. Vascular ACRs induced by long days were highly enriched in binding sites for flowering-related transcription factors. Among the highly ranked genes (based on chromatin and expression signatures in the vasculature), we identified TREHALOSE-PHOSPHATASE/SYNTHASE 9 (TPS9) as a flowering activator, as shown by the late flowering phenotypes of T-DNA insertion mutants and transgenic lines with phloem-specific knockdown of TPS9. Our cell-type-specific analysis sheds light on how the long-day photoperiod stimulus impacts chromatin accessibility in a tissue-specific manner to regulate plant development.
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