分辨率(逻辑)
显微镜
超分辨率
计算机科学
可视化
超分辨显微术
极限(数学)
纳米技术
生物物理学
物理
生物
光学
人工智能
材料科学
图像(数学)
扫描共焦电子显微镜
数学
数学分析
作者
Lothar Schermelleh,Alexia Ferrand,Thomas Huser,Christian Eggeling,Markus Sauer,Oliver Biehlmaier,Gregor P. C. Drummen
标识
DOI:10.1038/s41556-018-0251-8
摘要
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.
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