脉孢菌
粗脉脉孢菌
生物钟
黄素组
生物
隐色素
生物物理学
PAS域
转录因子
昼夜节律
黄素腺嘌呤二核苷酸
黄素单核苷酸
嗜光蛋白
细胞生物学
光敏色素
突变体
生物化学
植物
辅因子
红灯
酶
基因
神经科学
作者
Qiyang He,Ping Cheng,Yuhong Yang,Lixing Wang,Kevin H. Gardner,Yi Liu
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2002-08-02
卷期号:297 (5582): 840-843
被引量:387
标识
DOI:10.1126/science.1072795
摘要
Blue light regulates many physiological processes in fungi, but their photoreceptors are not known. In Neurospora crassa, all light responses depend on the Per-Arnt-Sim (PAS) domain-containing transcription factor white collar-1 (wc-1). By removing the WC-1 light, oxygen, or voltage domain, a specialized PAS domain that binds flavin mononucleotide in plant phototropins, we show that light responses are abolished, including light entrainment of the circadian clock. However, the WC-1-mediated dark activation of frq remains normal in this mutant, and the circadian clock can be entrained by temperature. Furthermore, we demonstrate that the purified Neurospora WC-1-WC-2 protein complex is associated with stoichiometric amounts of the chromophore flavin-adenine dinucleotide. Together, these observations suggest that WC-1 is the blue-light photoreceptor for the circadian clock and other light responses in Neurospora.
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