Penicillium expansum is one of the main pathogens causing decay in fruits and vegetables in recent years, researchers have discovered that some yeasts have antagonism against Penicillium expansum. In this study. two species of yeast that can biocontrol apple diseases caused by Penicilliurn expanurn were discovered At the same time, the antagonistic conditions such as yeast concentration, concentration of Fe2 in the yeast culture medium, and inoculation time were preliminarily studied, and the best conditions for yeast antagonism were determined.