生存素
肽核酸
核酸酶
核酸
化学
DNA
分子生物学
肽
癌症
生物物理学
癌症研究
生物
生物化学
细胞凋亡
遗传学
作者
Minghai Ma,Pu Zhang,Xiao Liang,Daxiang Cui,Qiuya Shao,Haibao Zhang,Mengzhao Zhang,Tao Yang,Lu Wang,Nan Zhang,Minxuan Jing,Lu Zhang,Weichao Dan,Rundong Song,Xi Liu,Jiatao Hao,Yuhang Chen,Lijiang Gu,Lei Wang,Jinhai Fan
出处
期刊:Nano Research
[Springer Nature]
日期:2021-09-04
卷期号:15 (3): 2278-2287
被引量:11
标识
DOI:10.1007/s12274-021-3807-z
摘要
One of the critical problems in bladder cancer (BC) management is the local recurrence of disease. However, achieving the accurate delineation of tumor margins intraoperatively remains extremely difficult due to the lack of effective tumor margin recognition technology. Herein, survivin molecular beacon (MB) and R11 peptide-linked spherical nucleic acids (SNAs) were synthesized as nanoprobes (AuNP-MB@R11) for sensitive detection of BC margins. Physicochemical properties proved that R11 peptides and survivin MB were successfully loaded onto the surface of SNAs. AuNP-MB@R11 had good stability against nuclease activity and high sensitivity and specificity to detect survivin single strand DNA (ssDNA) in vitro. According to cytology, R11 peptides could increase the BC targeting ability and membrane penetrability of SNAs. Notably, R11 peptides significantly promoted the disintegration of lysosomes and the release of SNAs to enhance fluorescence imaging quality. Further RNA sequencing proved that some genes and pathways related to endocytosis and lysosomes were significantly regulated, such as AGPAT5, GPD1L, and GRB2. In orthotopic BC models and a clinical sample from a patient with BC, AuNP-MB@R11 showed a more legible cancerous fluorescence margin and offered remarkably improved detection compared to those achieved by SNAs. R11 peptide-linked SNAs present promising potential to identify BC margin, which may help to improve the R0 resection rate in surgery and improve patients’ quality of life.
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