Objective: To compare two staining methods.Methods: Two staining methods were used to stain tissue sec-tion and cell smear respectively,the preparation methods and the staining effects of two staining methods were evaluated.Results: The modified staining buffer has less sediment,no oxidized membrane,don't need filtration,has shorter staining time and better staining effects.Conclusion:The modified staining method is better than the old method and can result in better staining effects.