Study on the Biological Characteristics of Nine Cordyceps militaris Strains

The biological characteristics of nine Cordyceps militaris strains were studied.The ITS sequence of the nine strains were analyzed and the cordycepin concentration in the mycelium and fermentation broth were also determined.The results showed that strain‘GIM 5.502',‘GIM 5.512',‘GIM 5.270',‘GIM 5.272',‘GIM 5.266'could change color in mycelium normally,but they could not develop fruit body regularly,the other four strains could neither change color in mycelium normally,nor form fruit body.The ITS sequence analysis showed that it had little change between different Cordyceps militaris strains.The cordycepin concentration in the mycelium and fermentation broth of the strain‘GIM5.502'were the highest among that of the other strains,which was up to 6.971mg/L,8.461mg/L respectively.However,the cordycepin could not be assayed in that of strain‘GIM 5.267',‘GIM 5.272'.