From the leaves of Ilex hainanensis Meer., a new triterpene glucoside, hainanenside (Ⅰ) was isolated beside the known rutin. On basis of MS, ~1HNMR and ~(13)CNMR as well as chemical evidences, the structure of its aglycone was found to be olean-12-ene 23 α, 28 β-dioic acid 3 β, 16 α-dihydroxyl (Ⅱ), (Ⅰ) is β-Dglycosyl (1′→28) ester of (Ⅱ).