In order to further increase the β-cyclodextrin production yield,different kinds of starch and preliminary treatment were investigated and compared.Among of them,corn starch was suitable for production.Furthermore,the effects of the size of starch,the damage extent of crystalline structure and the inhabitance of micromolecular sugar was investigated,and the results predict that CGTase was suitable for preferred gelatinized starch,but do not be used in industry production due to the fact that high viscosity.then a novel predisposal method was suggested and described as follows:heated at 85 ℃ for 1h,agitation for 24 h at the constanttemperature of 65 ℃.With the optimum conditions,a highest transformation yield achieved at 29.86%.The developed method was avoiding the problem of high conglutination caused by gelatinized starch and the micromolecular sugar caused by enzymed starch.