Effect of total flavone of Abelmoschus manihot(L.) Medic. on angiogenesis in human umbilical endothelial cells

Objective To investigate the proangiogenic effects of total flavone of Abelmoschus manihot(L.) Medic.(TFA) in human umbilical vein endothelial cells(HUVEC).Methods The HUVEC cultured in vitro served as the model to determine the cell proliferation with MTT.The effect of TFA on HUVEC migration was detected by Transwell chamber migration assay,and cell differentiation was examined by endothelial tube formation assay.Results Treatment with 5-20 μg/ml TFA for 72 h had a marked effect on the proliferation of HUVECs,and strongly promoted the migration,10-20 μg/ml TFA could promote tube formation of HUVEC.Conclusion TFA at certain concentrations exerts a potent proangiogenic effect on HUVEC in vitro.