软骨发生
3D生物打印
脚手架
关节软骨修复
再生(生物学)
生物医学工程
材料科学
软骨
组织工程
丝素
明胶
自愈水凝胶
活力测定
化学
复合材料
细胞
丝绸
关节软骨
骨关节炎
解剖
高分子化学
细胞生物学
医学
生物化学
替代医学
病理
生物
作者
Jingjie Shen,Wenjing Song,Jia Liu,Xiaobo Peng,Zhiqiang Tan,Yingni Xu,Sa Liu,Li Ren
标识
DOI:10.1016/j.ijbiomac.2023.127671
摘要
Cartilage has a limited ability to repair itself, highlighting the urgent need for suitable materials for cartilage regeneration. Achieving a balance between cell survival environment and hydrogel crosslinking density is crucial for photosensitive cell-laden cartilage repair materials to achieve both high strength and good cell viability. Here, an interpenetrating hydrogel consisted of methacrylate gelatin (GelMA) and glycidyl methacrylate silk fibroin (SG) was introduced. Compared to GelMA hydrogel, GelMA/SG had desired mechanical properties, with achieving up to 5 times of compression modulus and 6 times of compression failure energy. Meanwhile, the chondrocytes inside GelMA/SG exhibited great viability which was over 90 %. GelMA/SG as a bioink had favorable printability for digital light processing (DLP) bioprinting. The mesh DLP-printed scaffolds with high precision were created and GelMA/SG had a better shape retention ability than GelMA. Moreover, GelMA/SG cell-laden scaffolds had high strength while chondrocytes proliferated significantly in vitro culture. They were implanted under the skin of nude mice to evaluate ectopic chondrogenesis in vivo. The GelMA/SG cell-laden scaffolds indicated little deformation and high expression of collagen type II and glycosaminoglycans, which was advantageous for cartilage regeneration. The scaffold and its fabrication strategy provide potential solutions for clinical cartilage repair problems in the future.
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