类胡萝卜素
抗菌剂
抗菌肽
化学
铜绿假单胞菌
微生物学
肽
生物膜
金黄色葡萄球菌
抗菌活性
大肠杆菌
肺炎克雷伯菌
抗生素
细菌
生物化学
生物
遗传学
基因
作者
S. P. Sen,Ramkamal Samat,Moumita Jash,Satyajit Ghosh,Rajsekhar Roy,Nabanita Mukherjee,Surojit Ghosh,Jayita Sarkar,Surajit Ghosh
标识
DOI:10.1021/acs.jmedchem.3c01150
摘要
Antimicrobial cationic peptides are intriguing and propitious antibiotics for the future, even against multidrug-resistant superbugs. Venoms serve as a source of cutting-edge therapeutics and innovative, unexplored medicines. In this study, a novel cationic peptide library consisting of seven sequences was designed and synthesized from the snake venom cathelicidin, batroxicidin (BatxC), with the inclusion of the FLPII motif at the N-terminus. SP1V3_1 demonstrated exceptional antibacterial effectiveness against Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Klebsiella pneumoniae and destroyed the bacteria by depolarizing, rupturing, and permeabilizing their membranes, as evident from fluorescence assays, atomic force microscopy, and scanning electron microscopy. SP1V3_1 was observed to modulate the immune response in LPS-elicited U937 cells and exhibited good antibiofilm activity against MRSA and K. pneumoniae. The peptide promoted wound healing and disinfection in the murine model. The study demonstrated that SP1V3_1 is an exciting peptide lead and may be explored further for the development of better therapeutic peptides.
科研通智能强力驱动
Strongly Powered by AbleSci AI