In Situ Generation of Pyroptosis Inducer Mediated by Intracellular Labile Copper Pool for Safe and Robust Antitumor Immunotherapy

levels in cells, implementing the in situ synthesis of a pyroptosis inducer through the formation of catalytic nanoparticles with peroxidase (POD) and superoxide dismutase (SOD)-mimicking activities. Our results confirmed that the nanoplatform could generate high levels of ROS, resulting in pyroptotic cell death. When combined with antiprogrammed death receptor 1 therapy (αPD-1), the pyroptosis inducer exhibited excellent antitumor capacity in tumor models. Meanwhile, it exhibited minimal toxicity to healthy tissues due to the low intracellular copper concentration in normal cells. Overall, our work provides potential for the development of efficient and safe antitumor immunotherapy.