A UHPLC-Q-TOF-MS-based serum and urine metabolomics approach reveals the mechanism of Gualou-Xiebai herb pair intervention against atherosclerosis process in ApoE-/- mice

葡萄糖醛酸盐 化学 代谢组学 草本植物 代谢途径 生物化学 新陈代谢 葡萄糖醛酸化 药理学 代谢物 脂质代谢 色谱法 传统医学 医学 微粒体 草药
作者
Yuting Wang,Xin Sun,Jingwen Qiu,An Zhou,Peng-Bo Xu,Yarong Liu,Hongfei Wu
出处
期刊:Journal of Chromatography B [Elsevier BV]
卷期号:1215: 123567-123567 被引量:9
标识
DOI:10.1016/j.jchromb.2022.123567
摘要

Atherosclerosis (AS) is a metabolic disorder commonly correlated with a high-fat diet (HFD). There are many endogenous metabolic changes associated with AS development. Gualou-Xiebai (GLXB) is a traditional Chinese medicine herb pair that has been used to treat AS. However, the mechanism of GLXB herb pair on the process of AS is still essentially unknown. In this study, aortic histopathological examination and biochemical analyses were used to validate the anti-atherosclerotic effects of GLXB herb pair on ApoE-/- mice during the disease course of AS. The mechanism of GLXB herb pair were performed by metabolomics approach based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). As a result, GLXB herb pair has protective effects on AS lesion development and improves blood lipid levels in ApoE-/- mice. A total of 34, 39, and 49 metabolites were found to be profoundly altered in the 9-week, 14-week, and 19-week model groups compared with the corresponding control groups. Among them, 16, 18, and 18 metabolites showed a trend toward normal levels after pharmacological intervention. Metabolic pathway analysis found that GLXB herb pair mainly affects glycerophospholipid metabolism, pentose and glucuronate interconversions in 9 weeks; linoleic acid metabolism, cysteine and methionine metabolism, and arachidonic acid metabolism in 14 weeks; arachidonic acid metabolism and pentose and glucuronate interconversions in 19 weeks. The results demonstrated that GLXB herb pair mainly played a therapeutic role by regulating glycerophospholipid metabolism and pentose and glucuronate interconversions in the whole process of AS.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Kawhi发布了新的文献求助10
1秒前
朱瑶君完成签到,获得积分10
1秒前
zenmefeishi发布了新的文献求助10
2秒前
2秒前
科研通AI5应助温柔的海安采纳,获得10
3秒前
岳莹晓完成签到 ,获得积分10
4秒前
简单小刺猬完成签到 ,获得积分10
4秒前
wu发布了新的文献求助10
8秒前
8秒前
11秒前
TU发布了新的文献求助10
13秒前
现代鱼发布了新的文献求助10
13秒前
量子星尘发布了新的文献求助10
14秒前
Kawhi完成签到,获得积分10
14秒前
14秒前
16秒前
Hu完成签到,获得积分10
17秒前
18秒前
爱河发布了新的文献求助10
18秒前
欢喜大地发布了新的文献求助10
18秒前
18秒前
ding应助YanuoK采纳,获得10
21秒前
21秒前
wind完成签到,获得积分10
22秒前
eulota发布了新的文献求助10
22秒前
科研刘完成签到 ,获得积分10
22秒前
啰啰完成签到 ,获得积分10
24秒前
25秒前
25秒前
dmm发布了新的文献求助10
25秒前
26秒前
26秒前
27秒前
大模型应助科研通管家采纳,获得10
27秒前
斯文败类应助科研通管家采纳,获得10
27秒前
Orange应助科研通管家采纳,获得10
27秒前
我是老大应助科研通管家采纳,获得10
27秒前
wanci应助科研通管家采纳,获得10
27秒前
FashionBoy应助科研通管家采纳,获得10
27秒前
NexusExplorer应助科研通管家采纳,获得10
27秒前
高分求助中
(应助此贴封号)【重要!!请各位详细阅读】【科研通的精品贴汇总】 10000
Voyage au bout de la révolution: de Pékin à Sochaux 700
血液中补体及巨噬细胞对大肠杆菌噬菌体PNJ1809-09活性的影响 500
Methodology for the Human Sciences 500
First Farmers: The Origins of Agricultural Societies, 2nd Edition 500
Simulation of High-NA EUV Lithography 400
International socialism & Australian labour : the Left in Australia, 1919-1939 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4325279
求助须知:如何正确求助?哪些是违规求助? 3840252
关于积分的说明 12003814
捐赠科研通 3481134
什么是DOI,文献DOI怎么找? 1909404
邀请新用户注册赠送积分活动 954473
科研通“疑难数据库(出版商)”最低求助积分说明 855707