Laminin-511 and recombinant vitronectin supplementation enables human pluripotent stem cell culture and differentiation on conventional tissue culture polystyrene surfaces in xeno-free conditions

Human pluripotent stem cells can be successfully cultured for long passages on uncoated tissue culture polystyrene (TCP) dishes in xeno-free medium supplemented with optimal ratio and concentration of laminin-511 and recombinant vitronectin.