生物
葡萄
植物
微生物群
寡养单胞菌
叶圈
葡萄酒
生长季节
物候学
链格孢
园艺
假单胞菌
细菌
生物信息学
遗传学
食品科学
作者
Dániel G. Knapp,Anna Lázár,Anna Molnár,Balázs Vajna,Zoltán Karácsony,Kálmán Zoltán Váczy,Gábor M. Kovács
标识
DOI:10.1111/1758-2229.12950
摘要
Grapevine (Vitis vinifera) is a reservoir of fungal endophytes that may affect its growth, health status and grape production. Although there is growing interest in comparing fungal communities of mainly red grape varieties across various factors using only high-throughput sequencing, the small-scale mycobiome variations in geographically close vineyards need further examination. We aimed to characterize the fungal microbiome of the above-ground tissues of V. vinifera cv. Furmint in different plant parts, seasons and sites using culture-dependent and culture-independent methods, and in planta fluorescent microscopic visualization techniques. Samples were collected from four sites of the Tokaj wine region in Mád and two reference sites in Eger, Hungary, across different seasons for 2 years. Fungal endophytes of young and mature leaves, flowers and grape bunches were collected at different phenological stages. Based on each technique, Aureobasidium pullulans, Cladosporium spp. and the complex species Alternaria alternata dominated the community at every site, season and plant organ. We found no significant difference among communities in distinct neighbouring vineyards, nor when compared with the distant reference sites. We can conclude that the different shoot parts of the Furmint grapevines harbour a common core group of fungal community in these regions.
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