苏云金杆菌
Cry1Ac公司
菜蛾
甘露糖
生物杀虫剂
拉伤
多糖
阿拉伯糖
生物化学
半乳糖
微生物学
化学
细菌
生物
食品科学
转基因作物
植物
生殖器鳞翅目
杀虫剂
发酵
基因
木糖
解剖
转基因
遗传学
农学
作者
Meiling Wang,Geng Li,Bai Xue,Zeyu Wang,Wenyue Xu,Changlong Shu,Jie Zhang
标识
DOI:10.1016/j.ijbiomac.2021.08.193
摘要
Bacillus thuringiensis (Bt) are entomopathogenic bacteria that produce different kinds of insecticidal proteins. However, studies on Bt exopolysaccharides are lacking. Here, we aimed to explore the characteristics and insecticidal synergism of EPS, an exopolysaccharide from Bt strain 4D19. The molecular weight of EPS-2 was 58.0 kDa, which consisted of mannose (44.2%), GlcN (35.5%), D-GalN (8.0%), glucose (5.5%), arabinose (5.1%), galactose (0.9%), Man-UA (0.3%) and Glc-UA (0.2%). The toxicity of insecticidal proteins against Plutella xylostella was increased by adding EPS. EPS-2 bound to Cry1Ac protoxin and promoted the binding of Cry1Ac protoxin to the gut membrane of P. xylostella, but did not bind to activated toxins. These results suggested that EPS-2 may bind to the protoxin C-terminal region to enhance insecticidal activity. Our findings indicated that Bt strains produce exopolysaccharide to enhance the toxicity of insecticidal crystal proteins, which could be applied in biopesticide research and product development.
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