Clostridium vitabionis sp. nov., isolated from the large intestine of a mini-pig

An obligately anaerobic, Gram-stain-negative, spore-forming, short rod-shaped bacterium, designated strain YH- T4B42 T , was isolated from the large intestine of a mini-pig. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium aminophilum KCTC 5424 T , Clostridium symbiosum KCTC 15329 T and Clostridium butyricum KCTC 1871 T , with 95.5, 92.4 and 83.0 % sequence similarity, respectively. The average nucleotide identity values for strain YH-T4B42 T and the closest related strains were lower than 72 %. The G+C content of the isolate was 55.8 mol%. The cell-wall peptidoglycan was A1γ type and contained meso -diaminopimelic acid. The predominant fatty acids were C 16 : 0 , C 18 : 1 cis 9, C 14 : 0 and C 18 : 0 . The major end products of glucose fermentation were lactate, formate and acetate, with a minor amount of butyrate. Based on its phenotypic, phylogenetic and chemotaxonomic properties, a novel species, Clostridium vitabionis sp. nov., is proposed for strain YH-T4B42 T (=KCTC 25105 T =NBRC 114767 T ).