Establishment of three types of bladder cancer models with MB49 cells

Objective To establish three types of bladder cancer models with MB49 cells and investigate the optimal opportunity for experimental treatment. Methods The subcutaneous model of bladder cancer was established with 2×106 cells/50 uX MB49 cells;orthotopic model with 5×105 cells/50 μlMB49 cells through catheter, and pulmonary metastatic model with 1×105 cells/200 μl MB49 cells via tail vein. The optimal opportunity for experimental treatment was chosen. Results It was at the 5th day after transplantation that palpable tumor could be detected in subcutaneous model, which was the optimal opportunity for experimental treatment. The steady orthotopic model could be established by cancer cell bladder perfusion. Fist to third day was the optimal opportunity for experimental treatment. The observation duration was 3 weeks. The pulmonary metastatic model could be established by trail intravenous injection. The optimal time for experimental treatment was within 7 days. The observation duration was also 3 weeks. Conclusion The subcutaneous model, orthotopic model and pulmonary metastatic model of bladder cancer could be established by MB49 cells. The quality of the models wap good, and the time of tumor development was steady. It was favorable for choosing the optimal time of e Derimental treatment. Key words: Carcinoma of bladder; Model, animal