纳米点
荧光
电子显微镜
材料科学
显微镜
分辨率(逻辑)
荧光显微镜
纳米技术
对偶(语法数字)
生物物理学
光学
物理
生物
计算机科学
人工智能
艺术
文学类
作者
Navneet C. Verma,Chethana Rao,Ashutosh Singh,Neha Garg,Chayan Kanti Nandi
出处
期刊:Nanoscale
[Royal Society of Chemistry]
日期:2019-01-01
卷期号:11 (14): 6561-6565
被引量:10
摘要
Due to their high biocompatibility and nontoxic nature, carbogenic fluorescent nanodots (FNDs) have already shown their application in bioimaging. However, their non-specific labeling has restricted their application in live cell super resolution microscopy (SRM). Here we introduce, for the first time, an orange emissive FND, specifically conjugated to the HeLa cell actin filament, for successful single molecule stochastic optical reconstruction microscopy (STORM) and super resolution radial fluctuation (SRRF) microscopy. The resolution obtained in SRRF (∼35 nm) was almost an order of magnitude less than the diffraction limited spot. Interestingly, in addition, the FND also showed electron microscope (EM) contrast inside the cell. We hope that this FND will not only replace some of the common dyes used for SRM, but will also be used as a dual responsive marker in correlative super resolution microscopy (CLEM).
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